Professor
Torben Barington
OPEN - Odense Patient data Explorative Network
| Projekt styring | ||
| Projekt status | Active | |
| Data indsamlingsdatoer | ||
| Start | 01.01.2012 | |
| Slut | 01.01.2020 | |
Antibody repertoires and rearrangement mechanisms elucidated through next generation sequencing
Short summary
The purpose is to elucidate basic mechanisms involved in the generation of antibody specificity by sequencing large numbers of rearranged immunoglobulin genes (so called VDJ genes) and subjecting them to bioinformatics analyses.
Rationale
Human antibodies are formed by cells expressing a unique VDJ gene assembled by gene rearrangements in the bone marrow and later modified by somatic hypermutation in the lymph nodes. High throughput sequencing is a means to study the mechanisms behind these phenomena in detail. In this project, B cells from selected patients as well as from normal individuals are sequenced and bioinformatics tools developed allowing inference of basic mechanisms behind antibody production as well as pathogenic mechanisms involved in selected immunodeficiencies.
Description of the cohort
B cells have been purified from blood samples from 120 healthy blood donors, anonymized and pooled. DNA was then extracted and subjected to PCR amplification and next generation sequencing of rearranged VDJ and DJ genes. These sequences were supplemented by publicly available VDJ sequences from the GeneBank database.
Data and biological material
A total of 43,161 sequences including 34,100 VDH and 9,061 DJ sequences are available for analysis.
Collaborating researchers and departments
Department of Clinical Immunology, Odense University Hospital
- Tina Funck
- Mike Bogetofte Barnkob
- Nanna Holm
- Torben Barington
- Tina Funck
MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxfordshire
- Mike Bogetofte Barnkob
- Line Ohm-Laursen
- Camilla Slot Mehlum