OPEN Research Support
head

Physician
Shakespeare Jeromdesella
KMEB, department of Endocrinology, OUH


Projekt styring
Projekt status    Open
 
Data indsamlingsdatoer
Start 01.12.2021  
Slut 01.12.2024  
 



Studies on effects of age and diseases on cellular heterogeneity of human bone-marrow stromal stem cells

Short summary

The aim of this project is to determine Bone marrow stromal (skeletal) stem cells (BM-MSCs) subpopulations molecular properties, biological features, osteogenic differentiation capacity along with their spatial anatomical localization in vivo. This study investigates the gender-related, age-related and osteoporosis related changes in BM-MSC heterogeneity.


Rationale

Osteoporosis is one of the most serious threats to public health in the industrialized world. The risk of a person getting an osteoporotic fracture during his life is 40-50% for women and 13-22% for men. These fractures are associated with serious health complications and economic consequences for society. Bone marrow stromal cells are multipotent stem cells with a finite replicative capacity. During their proliferative state, they can differentiate into mainly osteoblasts, chondrocytes and adipocytes. During the aging process, their ability to proliferate and differentiate becomes impaired, affecting their function and contributing to age-related pathologies such as osteoporosis. Blood biomarkers can be associated with the bone aging process and can also help assess the risk of bone fractures in patients with osteoporosis, but to understand what drives the aging process at the cellular level in the first place, it is imperative to characterize the cellular heterogeneity found among the cells. This knowledge would help to distinguish the molecular signatures among different individuals with different ages and metabolic state and provide information to reduce the cellular heterogeneity of the cells used for therapy with the aim of enhancing their therapeutic potential.


Description of the cohort

10 healthy males and females 20-35 years old 10 healthy males and females 65-80 years old 40 patients with Osteoporosis


Data and biological material

bloodsamples, bonemarrow aspirate and bone biopsi.